Histological studies of muscle tissue in swine sarcocystosis in the northern Kazakhstan

Аннотация

Background and Aim. At present there is no information about the prevalence of sarcocysto-sis among pigs in Kazakhstan, so we aimed to study pork meat for the presence of sarcocysts in the muscles of pigs in the Kostanay region.

Materials and Methods. Pieces of heart muscle, neck, oesophagus, and diaphragm legs taken from pig carcasses served as material for the study. Sarcocysts presence in muscle slices was deter-mined by viewing the samples stained with methylene blue under a microscope. Morphological analysis of muscle tissues included histological and histochemical methods.

Results. Muscle samples from 71 pig carcasses were examined. The intensity of sarcocystosis in pigs was 42.2%, and the intensity of invasion was 8.43 cysts. The highest infection with sarco-cysts was found in sows 3-5 years old (29.6%). The predominant sarcocysts localisation was found: in the heart - 23.9%, oesophagus - 12.7%, and the diaphragm legs - 5.6%. Cervical muscles from the same animals were free of sarcocysts. Sarcocystis suicanis species was detected Pathological chang-es in muscle fibres were detected in the examined muscle slices. Swellings and inflammatory pro-cesses of focal or diffuse character, as well as serous and less often purulent reactive myositis with infiltration and admixture of eosinophils or lymphocytes were noted. Examination of slices revealed an immuno allergic reaction leading to disruption of the heart histological structure, the oesophagus fibres and the diaphragm legs.

Conclusion. Such animal muscles studies for sarcocystosis have not been conducted in the Kostanay region. The prevalence of infection from the number of the studied livestock were deter-mined. According to studies in animals, the infection prevalence increases with age, which is associ-ated with increased contact of pigs with primary hosts. According to the histological studies results, we have established inflammatory processes and muscle lesions caused by exposure to the product of parasite vital activity.