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Abstract

In order to ensure long-term storage of the genetic resources of apple, tested the effect of cryoprotectants, methods and timing of pre-treatment on the viability of winter dormant buds in cryopreservation in liquid nitrogen. It was found that the application of cryoprotectants PVS2 and 50% glycerin + 50% glucose increases the viability of apple winter buds in cryoconservation to 100%. The best way is pre-treatment by soaking in cryoprotectants for 3 hours after low-temperature acclimatization. The results of the research can be used to create cryobanks of fruit crops.