EFFICIENCY OF VARIOUS DNA EXTRACTION METHODS FROM OPISTHORCHIS FELINEUS
Keywords:
DNA isolation, PCR formulation, extracting buffer, CTA buffer, phenol-chloroform method, mature marit, molecular genetic diagnostics, trematode, opisthorchiasis, amplificationAbstract
The authors of the article provided results on the efficiency of DNA isolation from adult forms of O. felineus maritus. The results of exposure to various methods of DNA extraction are shown in the example of measuring the concentration in the obtained samples, while we saw that the purity and concentration of DNA generally depend on physical or chemical effects on trematodes. The resulting DNA was subsequently used to perform PCR analysis with specific primers of the ITS2 ribosomal (nuclear) genome. The results were detected on a 1% agarose gel, where on the electropherogram as can be seen by the intensity of amplicon output the most effective way to isolate DNA from adult forms of O. felineus. In the course of the research, the optimal solution concentrations were selected and modifications were made to all three methods for the best DNA yield. Also, a DNA isolation protocol was developed and a program was selected for the production of PCR analysis
