EVALUATION OF POTATO BREEDING MATERIAL FOR THE PRESENCE OF DNA MARKERS OF RESISTANCE TO POTATO X-VIRUS

Abstract

The article presents the results of the identification of molecular genetic markers linked to resistance genes to the potato X-virus (PVX) in four varieties and three potato breeding lines from the collection of KazATU named after. S. Seifullin, as well as in six potato hybrids obtained as a result of four crossing combinations. Identification of DNA markers by polymerase chain reaction was carried out.
The molecular marker GM637 (Nb gene) was identified in potato varieties Udovitsky and Aladin; molecular markers 5Rx1 and GM339 (genes Rx1 and Nb) in variety Xisen 6 were identified; in the breeding lines Z897-3 and Z872-3, the 5Rx1 marker (Rx1 gene) was determined; 5Rx1 and GM339 molecular markers (Rx1 and Nb genes) in the genotype of the Twostep variety were detected. In the study of six potato hybrids, it was revealed that all the studied hybrids inherited one molecular marker: in hybrids KC-1, KC-10 and KC-11, a molecular marker 5Rx1 (Rx1 gene) was detected, in hybrids KC-2, KC-15 and KC -23 molecular markers GM637 and GM339 linked to the Nb gene were detected. On the basis of the obtained results, it is recommended to use Xisen 6 and Tustep potato varieties as parental forms during breeding programs.