Using of immunochromatographic analysis to determine antibiotics in milk

Abstract

Background and Aim. Failure to comply with the rules for the use of antibiotics and/or the tim-ing of milk production from cows subjected to antibiotic therapy leads to the entry of residual amounts of drugs into the human body with dairy products, causing various pathologies. For practice, simple but sufficiently sensitive and specific rapid tests are needed to determine the safety of milk. Such tests can be developed based on immunochromatographic analysis (ICA).

Materials and Methods. Colloidal gold (CG) particles were prepared according to the method of Wang et.al. (2014) and examined using transmission electron microscopy and UV-visible spectroscopy. CG particles were used to label antibodies against streptomycin (STR), tetracycline (TC) and/or chloramphenicol (CAP) according to the method of Yakovleva et al. (2012). The ICA test was developed based on the principle of competition of antibiotics in milk with analogues immobilized on a nitrocellulose membrane.

Results. The multiplex ICA test showed sensitivity at the level of the best world analogues, detecting STR, TC and CAP in milk in concentrations equal to or exceeding the maximum residue limits (MRL) for these antibiotics: 200 ng/ml, 10 ng/ml, 0.3 ng/ml, respectively. The advantage of the ICA test is its affordability, since the proposed technologies for the production of antibiotic-specific anti bodies and immunoassay test line reagents significantly reduce the cost of domestic products, making them more accessible to the country's consumers.

Conclusion. A domestic ICA test system has been designed for simultaneous rapid analysis of milk for the content of streptomycin, tetracycline and chloramphenicol.