GENERATION OF MONOCLONAL ANTIBODIES AGAINST TRICHINELLA SPIRALIS AND DETERMINATION OF THEIR IMMUNOCHEMICAL PROPERTIES

Abstract

Diagnosis of trichinellosis in humans and animals is still in the developmental stage of the most effective and sensitive test for early detection. Serological methods are the most sensitive for detecting trichinella invasion. However, there is a high risk of non-specific reactions with other parasitological diseases. To address this issue, monoclonal antibodies (mAbs) against excretory-secretory antigens (ESAg) of trichinella muscle larvae are employed. In this study, hybridomas secreting mAbs against ES-Ag of Trichinella spiralis muscle larvae were generated. Out of 79 colonies, 5 mAb producer monoclonal clones (1D5, 1E7, 3C10, 4D6, 4F7) were obtained, and further stability testing selected 3 clones (1D5, 1E7, and 4F7) that yielded 14 to 40% active subclones. Western blotting using T. spiralis ES antigens demonstrated that the mAbs recognized a single band at 75 kDa. The productivity of the clones ranged from 2.0 to 4.0 mg/ml of protein. The obtained results indicate that the high activity, specificity of mAbs, and productivity of the clones recommend these mAbs for application in diagnostic purposes.