DETERMINATION OF IMMUNIZING DOSES OF THE COWPOX VACCINE CANDIDATE: PRELIMINARY RESULTS

Abstract

Determining the effective immunizing dose of a vaccine is one of the important pillars of a vaccination strategy. In this regard, this article presents the results of studies to determine the effective immunizing dose of a candidate cowpox vaccine prepared from the BIEMG-51 strain of the vaccinia virus. At determining a immunizing dose of the vaccine, it was found that in all animals vaccinated at doses of 103 TCID50, 104 TCID50, 105 TCID50, antibodies were not detected in the serum neutralization test (SNT). However, these vaccinated calves at the indicated doses were resistant to the challenge, while the unvaccinated group responded to the challenge and fell ill with the characteristic clinical signs of cowpox. The obtained primary results give grounds for improving the technology of vaccine preparation and in-depth study of the factors of cellular immunity of vaccinated animals in case of poxvirus infections.