STUDYING THE DIAGNOSTIC VALUE OF RECOMBINANT CAMPYLO-BACTER JEJUNI ANTIGENS

Abstract

This article describes the results of a study on the diagnostic value of Campylobacter jejuni recombinant antigens, Campylobacter Omp18 protein and major outer membrane protein (MOMP). In the present study, these proteins were used as antigens in enzyme im-munoassays to detect specific antibodies in the serum of cattle. Commercial native protein antigens were used to compare the effectiveness with similar studies. In total, 95 blood se rum samples from cattle from various farms in the northern region of the Republic of Ka-zakhstan were used. The greatest number of positive results was observed when using a commercial native antigen (52.6%); 47.4% of sera reacted positively with respect to the Omp18 antigen, and 38.9% with respect to the MOMP antigen. Statistical analysis revealed a high correlation between the native and Omp18 antigens and, to a lesser extent, with MOMP32. Because native antigens contain many different cell-wall proteins, they are less specific. Therefore, for serological diagnosis of cattle using enzyme immunoassays, it is ad-visable to use the C. jejuni outer membrane recombinant antigen MOMP32, as it has valua-ble diagnostic properties.