OBTAINING MONOCLONAL ANTIBODIES SPECIFIC TO GLY-COPROTEINS ASSOCIATED WITH PREGNANCY
DOI:
https://doi.org/10.51452/kazatu.2021.2(109).809Keywords:
pregnancy diagnostics, glycoproteins associated with pregnancy, recombinant an-tigen PAG1, immunization, hybridization, cloning, monoclonal antibodies.Abstract
As a result of hybridization of immune splenocytes and cells of the myeloma line of mice, strains producing monoclonal antibodies to the epitopes of the recombinant PAG1 antigen were obtained. For further studies, a strain of hybrid cells with the author's name 3D9D9 was selected, the antibody titers of the culture fluid of this subclone of ELISA comprised from 1:3200 to 1:6400. A preparative amount of monoclonal antibodies was obtained by the in vivo method and their main immunochemical properties were studied. The titer of purified antibodies from ascites fluid was 1: 12800, they belong to class G immunoglobulins, subclass G1 and have an L chain of the kappa type. As a result of immunoblotting, the epitope orientation of MCA to antigenic determinants of recombinant PAG1 with a molecular weight of 57 kD was determined. Thus, it was proved that the obtained monoclonal antibodies have specificity for the antigens of the recombinant PAG1 and can be used in the design of a test for determining pregnancy.
