CONSORTIUM OF MICROORGANISMS USED FOR OBTAINING POLYFUNCTIONAL BIOPREPARATIONS WITH BIOLOGICAL ACTIVITY

Abstract

It is becoming more urgent to develop adequate microbiological preparations for agriculture, representing themselves as living cells of microorganisms. The study aimed to create a new multifunctional consortium of microorganisms with a high level of biological activity, adapted to deep cultivation and suitable for obtaining biological products. The article describes the stages and results of research on developing a new multifunctional consortium of microorganisms "UMBK-1T" with a high level of biological activity, characterized by the constancy of the composition of the population, adapted to submerged cultivation. The authors studied microorganisms' cultural and morphological properties, carried out metagenomic NGS sequencing, analyzed the genome, and selected storage methods for a consortium of microorganisms. Genetic analysis showed that the microbiome of the new probiotic preparation mainly consists of microorganisms of the Firmicutes type (96.42%). The dominant species in the community are L. hilgardii - 18.02%, L. camelliae - 15.24%, L.rhamnosus - 1.66and S. cerevisiae - 38.07%. The consortium of microorganisms UMBK-1T has been deposited in the Collection 14 of Microorganisms of the Republican State Enterprise "Republican Collection of Microorganisms" of the KN MES RK under the number RKM 0862.

The article describes the stages and results of research on the development of a new multifunctional consortium of microorganisms "UMBK-1T" with a high level of biological activity, characterized by a constant population composition adapted to deep cultivation.

The authors studied the cultural and morphological properties of microorganisms, conducted metagenomic NGS sequencing, analyzed the genome, and selected methods for storing a consortium of microorganisms. Genetic analysis showed that the microbiome of the new probiotic drug mainly consists of Firmicutes – type microorganisms (96.42%). The dominant species in the community are Lactobacillus hulgardii – 18.02%, Lactobacillus camelliae – 15.24%, Lactobacillus rhamnosus – 11.66% and Saccharomyces cerevisiae – 38.07%. The consortium of microorganisms UMBK-1T is deposited in the Collection of microorganisms of the RSE "Republican Collection of Microorganisms" of the KN MES RK under the number RKM 0862, reference 11LB-1Sc.

On the basis of a consortium of microorganisms, a probiotic drug has been developed to increase the efficiency of feed use in animal husbandry and normalize the work of the gastrointestinal tract of animals; a drug to increase the yield of indoor, agricultural and forest crops; a biological product for cleaning and separating oil sludge contamination.