THE RESULTS OF FORCED ADAPTATION OF THE CELLS OF THE TESTICLES AND KIDNEY LAMBS AND GOAT KIDS TO CONTINUOUS GROWTH IN VITRO
Keywords:
primary cells, transplanted cells, trypsinization, transplanting, morphology, proliferation, adhesion, passages, adaptation.Abstract
In studies for obtaining continuously transplanted cells culture - substrateproducer of the virus lumpy skin disease, a primary culture of cells from the tissues of the kidneys and testicles of lambs and goat kids was used. To adapt to growth under conditions of undeveloped in vitro, they went through certain numerical passages using multiple cultivation methods at 37°C. The results of passages and evaluation of cell populations in the process of cultivation showed that testicular cells have fibroblast-like morphology, a relatively large adhesive and proliferative activity. After 14 and 16 passages in testicular cells of lambs and goats proliferative activity decreased significantly. After further forced passages and cloning, two clone populations of testicular cells of lambs were obtained, which were characterized by increased proliferative activity. Further passages and adaptation to reproduction in vitro can allow to obtain the desired cell line.
