DEVELOPMENT OF EXPRESS TEST FOR DIAGNOSIS OF CAMPILOBACTERIOSIS OF CATTLE
Keywords:
As a result, an immunochromatographic test was developed to diagnose genital campylobacteriosis of cattle, which can be recommended for veterinary practice.Abstract
As a result of the research, the optimal methods for conjugating specific antibodies and colloidal gold were selected. The optimal parameters for the application of antibodies for the formation of detection zones were determined: "test zone" - 0.1 mg / ml, and control - 5 μg / ml. The optimal concentration of conjugate addition to the support is 10 μg / ml. The sensitivity threshold of the test
is 15 ng soluble antigen. The specificity of the test was determined, the interaction of the reagents occurs only with respect to the C. fetus venerealis antigen. Laboratory tests of the developed test were performed in comparison with the PCR-analysis method and the commercial ICA test. As a result of the study of these samples, in no case the presence of an agent of this infection was identified. Laboratory tests confirm the effectiveness of the developed test, since they can detect the presence of the pathogen within 15 minutes, without the use of additional equipment. As a result, an immunochromatographic test was developed to diagnose genital campylobacteriosis of cattle, which can be recommended for veterinary practice.
