MOLECULAR ANALYSIS OF SUGAR BEET SAMPLES FOR THE PRESENCE OF A RESISTANCE GENE TO BOLTING
Keywords:
sugar beet, bolting, PCR - analysis, DNA, molecular marker, primer, gene, hybrid, allele, electrophoresis.Abstract
In this article, bolting problems in sugar beet collection hybrids and lines of Kazakh Scientific Research Institute of Agriculture and Plant Growing were studied. The scientific works related to the issues of sugar beet bolting conducted in the world and Kazakhstan were analyzed. The needs for these studies were identified and justified. The results of the analysis of samples using the marker CAU3903b which is specific to the BR1 locus corresponding to the presence of the bolting gene. Using methods: DeLaporta (extraction of genomic DNA from the leaves), PCR analysis revealed that the 21 samples out of 32 of sugar-beet with amplificated DNA fragment size of 208 bp have a resistance gene to bolting. This 21 samples are recommended to breeders as a genetic source for further breeding work in order to shorten the time to develop new hybrids.
