EXPRESS PEST RISK ANALYSIS OF POTATO SPINDLE TUBER VIROID (POTATO SPINDLE TUBER VIROID, PSTVD) FOR THE TERRITORY OF THE REPUBLIC OF KAZAKHSTAN
DOI:
https://doi.org/10.51452/kazatu.2023.3(118).1440Keywords:
potato spindle tuber viroid; express pest risk analysis; diagnostics; reverse transcriptase polymerase chain reaction; plant quarantine; varieties and hybrids of potatoes.Abstract
The article gives the results of the analysis of potato varietal samples for the presence of a dangerous quarantine object for the territory of the Republic of Kazakhstan - potato spindle tuber viroid by а reverse transcriptase polymerase chain reaction with using six different pairs of primers. In the working collection of potato varieties and hybrids of the Non-Commercial Joint-Stock Company «S. Seifullin Kazakh AgroTechnical Research University» were revealed 1 sample in 2021 and 19 samples in 2022 of Kazakh and foreign selection with fragments having a molecular mass characteristic to the potato spindle tuber viroid.
Cases of potato spindle tuber viroid detection served as a signal for conducting an express pest risk analysis for the country. In accordance with EPPO Standard PM 5/5 (1) "Guidelines on pest risk analysis: decision-support scheme for an express pest risk analysis", a resume of the express pest risk analysis has been formed. The results of the analysis confirm the need to change the status of the quarantine facility in the country. The results of the research, formed in the resume, will be transferred to the appropriate plant quarantine authorities.
