APPROBATION OF ASQ TECHNOLOGY FOR GENOTYPING CHITINASE GENES SUGAR BEET SAMPLES

Abstract

In the beet-growing regions of Kazakhstan, due to the permanent cultivation of sugar beet, there is a high infection of soils with the accumulation of pathogenic
microorganisms that cause root rot. The solution to this issue is the creation and introduction of new sugar beet hybrids resistant to root rot caused by fungi of the genus
Fusarium. To solve the issues of selection of genotypes resistant to root rot, the introduction of highly effective DNA markers into the selection process of sugar beet
is relevant. The purpose of this study was to test an allele– specific polymerase chain reaction using ASQ technology on sugar beet plants for resistance to root rot. This
method of genotyping is simple, affordable and budget-friendly for use in practical plant breeding. The studies used SNP (Single Nucleotide Polymorphism) markers for
sugar beet acid chitinase genes (BvSE2, BvSP2) associated with resistance to root rot.
The material was 28 samples of sugar beet from the working collection of LLP «Kazakh Research Institute of Agriculture and Plant growing». As a result of the
studies, the optimal composition and mode of polymerase chain reaction for the BvSE2 marker was determined. 23 sugar beet samples, carriers of the homozygous resistant
aa allele of the BvSE2 chitinase gene, 4 samples with the homozygous sensitive bb allele and 1 sample with the heterozygous ab allele were identified. Polymerase chain
reaction optimization is still required for the BvSP2 molecular marker. The obtained optimization results for the BvSE2 gene can be applied in practical selection of sugar beet for mass genotyping for resistance to root rot.