EXTRACTION AND IDENTIFICATION OF POLYPHENOLIC COMPONENTS FROM SERRATULA CORONATA L. AND SALSOLA COLLINA PALL. USING VARIOUS TECHNOLOGICAL PARAMETERS

Abstract

The work investigated the shoot of Serratula coronata L. and Salsola collina Pall. The plants were collected on the territory of the Karaganda region in various phases of vegetating to determine the most optimal age of the taxon for the presence of biologically active polyphenols. To determine the optimal conditions for the extraction of active extractive substances and polyphenolic components from the plants, several types of solvents with minimal toxic effect were used: ethanol in various concentrations and 0.1 N sodium bicarbonate solution. The qualitative and quantitative content of the main flavonoids in the composition of the obtained extracts was determined by high-performance liquid chromatography (HPLC).

It was found that the most appropriate time period for collecting Serratula Coronata L. begins with the third decade of May and ends in the first half of June. Analysis of the degree of extractivity of plant raw materials showed that raw materials must be collected before the onset of increased air temperature. There is no time and weather limits to collect Salsola collina Pall plant raw materials, due to the fact that the habitat of this plant is arid places. However, the vegetative period was observed only in late May – early June, and the collection during this period is not productive due to the beginning of an accumulation of plant mass. Therefore, the beginning of July is the recommended time for collecting Serratula Coronata L.