ISOLATION OF LACTIC ACID BACTERIA, PROMISING AS STARTER CULTURES FOR THE DEVELOPMENT OF FERMENTS FOR DIRECT APPLICATION
Keywords:
lactic acid bacteria, Lactobacillus, Pediococcus, Enterococcus, Weissella, Saccharomyces, starter cultures, identification, 16S rRNA.Abstract
The object of this study was 36 lactic acid bacteria isolated from various fermented milk products. The purpose of the work was to isolate lactic acid bacteria that are promising as starter cultures for ferments of direct application and their identification for the development of starter cultures for direct application. A total of 36 lactic acid bacteria were isolated, 19 active isolates were selected for screening as a result of the screening. Phenotypical and genetic identification of LAB cultures was carried out. As a result of bacterial genotyping by analysis of the sequence of the 16S rRNA gene, the selected isolates were related to genera
Lactobacillus, species casei, paracasei, fermentum, curieae, oryzae; Pediococcus acidilactici, pentosaceus; Enterococcus, species of faecium, lactis, Weissella, species of confusa. Genetic identification based on the ITS region fragment showed that 2 cultures were identified as Saccharomyces cerevisiae and Saccharomyces lactis.
