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Abstract

The article presents the results of NGS-sequencing of flushing from the nasal cavity of a healthy colt. Microbiome regulates many vital processes of the body. This study is necessary for a detailed understanding of the processes occurring between microorganisms inhabiting a particular organ and their relationship to the cells of the body. Traditionally, the microbial population is studied through cultivation techniques, physical, chemical and biochemical tests. These methods are time-consuming, take a lot of time, require prior knowledge of microorganisms of interest for their isolation from the community, and are not as accurate as
identification by genotypic methods. A comprehensive study of the microbiome composition of individual organs and tissues of organisms became possible with the advent of new molecular genetic methods, in particular NGS-sequencing of the next generation (Next Generation Sequencing). The new technology of NGS-sequencing on the MiSeq Illumina platform, the DNA obtained directly from the flush was analyzed without the cultivation stage on nutrient media. Genetic identification of all bacteria present, including
noncultivated forms, was carried out. Genetic analysis showed that the microbiome of the foal's nose consisted of the following phylum: Firmicutes (73.42%), Actinobacteria (8.36%), Proteobacteria (8.33%), Cyanobacteria (6.69%), Bacteroidetes (1.55%%) and Fusobacteria (0.75%). The dominant species in the community was Lactobacillus equi (12.09%). The study of the microbial community of animal organs and tissues will help to understand the mechanism of their interaction between themselves and the
macroorganism, in normal and pathology. Knowledge of these processes will be useful in the development of drugs for the treatment and prevention of animal diseases.