Obtaining in vitro sterile seedlings from sea buckthorn seeds

Abstract

Background and Aim. Sea Buckthorn (Hippophae rhamnoides) is a dioecious shrub with deciduous leaves. Due to the high content of biological compounds in its leaves and berries, this plant is currently known as a remedy used to maintain the health of humans and animals. The purpose of the work is the introduction of sea buckthorn plants into culture in vitro, the choice of sterilization modes and the composition of the nutrient medium.

Materials and Methods. Sea buckthorn seeds were used as the material for the study. Various antiseptics were used for sterilization: ethanol, mercury (II) chloride, hydrogen peroxide and sodium hypochlorite. The sterilization time for each antiseptic was selected experimentally. Various nutrient media and growth regulators were used for the growth of sterile sprouts. Activated carbon was used as an antioxidant to suppress the release of phenolic compounds.

Results. The largest percentage of sterile and viable explants (83.3%) was obtained using a 0.1% mercury (II) chloride solution. The proliferation of explants depended both on the composition of the nutrient medium and on the varietal characteristics of sea buckthorn. The maximum shoot length was 4.0 cm for the Sh-9-81(4-5) variety on a medium with 1.0 mg/l VAR, 5.0 mg/l IAA and 2 g/l activated carbon.

Conclusion. The growth rate depended on the variety and the composition of the nutrient medium. In the Sh-9-81(4-5) variety, the maximum shoot length (4.0 cm) was determined in a medium containing 1.0 mg/l BAP, 5.0 mg/l IAA and 2 g/l activated carbon. We recommend a medium containing 1.0 mg/l BAP, 5.0 IAA and 2.0 g/l activated carbon as an effective medium for the proliferation of Hippophae rhamnoides shoots.