SALT-TOLERANT MICROORGANISMS ISOLATED FROM COAL AND THEIR ABILITY TO STIMULATE WHEAT GERMINATION AND GROWTH

Abstract

The article describes the isolation and identification of bacteria, fungi, and actinomycetes from low-grade coal from the Kumiskuduk and Kuznetsk coal deposits in the Karaganda region. A total of 28 microbial isolates were obtained, and from these, 10 strains that showed stable growth on Burke's nutrient medium with 1% sodium chloride were selected: Bacillus firmus  №2, Microbacterium ginsengiterrae №4, Microbacterium ginsengiterrae №5, Brevundimonas vesicularis  № 11, Brevundimonas nasdae  №12, Brevundimonas vesicularis №19, Pseudomonas mandeliі  №25, Streptomyces parvus  №26, Streptomyces parvus №27 and Brevundimonas vesicularis №28. Laboratory studies showed that treating wheat seeds with metabolites from strains Bacillus firmus №2, Microbacterium ginsengiterrae  №4, Brevundimonas nasdae №12, Brevundimonas vesicularis №19, Pseudomonas mandelii  №25, Streptomyces parvus №27 and Brevundimonas vesicularis №28 resulted in higher germination rates and germination energy compared to the control. Additionally, strains  Bacillus firmus №2, Microbacterium ginsengiterrae №4, Brevundimonas nasdae №12 and Brevundimonas vesicularis №19 stimulated wheat seedling growth by 8.4–12%, and strain Brevundimonas vesicularis №19 increased root length by 5.8% compared to the control. Strains Bacillus firmus  №2, Microbacterium ginsengiterrae №4, Brevundimonas nasdae №12 and Brevundimonas vesicularis  №19 are recommended for the development of biopreparations for the bioremediation of saline soils and as growth stimulants for cereal crops. The entire content has been translated into English, and nothing has been left untranslated.