IN VITRO POTATO VARIABILITY AND ITS EFFECT ON TUBER NUTRITIONAL PROPERTIES

Авторы

  • Gajimuradova A. M. S. Seifullin Kazakh Agrotechnical Research University
  • Kirgizova I. V. K. A. Timiryazyev Russian State Agrarian University– Moscow Agricultural Academy
  • Silayev D. V. «National Center for Biotechnology» LLP
  • Turpanova R. M. «L.N.Gumilyov Eurasian National University» NCJSC
  • Ismukanova G. Zh. «L.N.Gumilyov Eurasian National University» NCJSC
  • Shvidchenko V. K. «North Kazakhstan Experimental Agriculture Station LLP

DOI:

https://doi.org/10.51452/kazatu.2023.2(117).1417

Ключевые слова:

potatoes; Solanum tuberosum; in vitro culture; somaclonal varia-bility; starch; total protein; free sugars.

Аннотация

A comparative analysis of nine somaclonal Dutch selection “Alladin” potato variants test results was carried out using a modified Murashige-Skoog medium with different concentrations of hormones, vitamins and growth stimulants. As a result of the work carried out, 100% callus formation providing from leaf explants and more than 90% shoot formation without the use of additional steps associated with their rooting optimal protocols for in vitro cultivation of potatoes were defined. According to morphological characteristics 9 initial lines were selected among the Dutch selec-tion of “Alladin” potato variety regenerated plants using these protocols. The tubers of the second reproduction obtained from these lines were used for the analysis of bi-ochemical parameters (starch, soluble sugars, proteins). As a result, lines with the maximum starch content were identified, 2 lines out of 9 exceeded the control sample by 3.3% and 10.7%. The protein content in the tubers of 7 somaclonal variants from 1% to 10.6% exceeded this indicator in the control. In comparison to the control, the sugar content was significantly lower (from 37.9 to 54%) in 3 studied samples. The results obtained are of great practical interest for further breeding studies to consoli-date them and create new dietary and table varieties of potatoes. Based on the ob-tained somaclonal variants, new promising potato varieties adapted to the soil and climatic conditions of Northern Kazakhstan can be obtained.

Биография автора

Silayev D. V., «National Center for Biotechnology» LLP

Кандидат медицинских наук старший

научный сотрудник ТОО  «Национальный

центр биотехнологии»

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Опубликован

2023-06-26