Optimization of the protocol for PCR-diagnosis of Potato tuber spindle viroid (Potato spindle tuber viroid, PSTVd)
DOI:
https://doi.org/10.51452/kazatu.2025.3(127).2009Abstract
Abstract
The article presents a brief characterization of PCR diagnostics of potato tuber spindle tuber viroid (hereinafter - PSTVd, viroid) samples found on the territory of the Republic of Kazakhstan. The selection of primers PSTVd-for-XbaI, PSTVd-rev-BamHI, PS88M, PS89P, PS7P, PS7M, Vir1, Vir2 specific to VVCC was performed. The results of diagnostics of domestic and foreign samples by reverse transcriptase polymerase chain reaction (hereinafter - RT-PCR) are presented. The temperature gradient of primer annealing for OT-PCR was studied.
Keywords: potato tuber spindle viroid; diagnosis; quarantine object; reverse transcriptase polymerase chain reaction; primer; sequencing.
Published
2025-09-30
Issue
Section
Agricultural sciences