-

Abstract

The article presents the results of a study aimed at finding the optimal conditions long-term storage of cultures of microorganisms with iron-sulfuroxidizing properties and that are involved in the process of bioleaching of metals. The main methods of research is cryopreservation and lyophilization using protective environments based on pyrite. We apply these protective components
such as gelatin, sucrose, glycerol, polivinilpiralidon, ascorbic acid, horse serum. All protective environment effective. Viability index is 1010, which remains stable during storage. At the same morphological and cultural characteristics remain unchanged, foreign microflora contamination does not occur. These results have implications for the development of the collection of the case and will be applied in practice in the Republican collection of microorganisms.