A STUDY OF THE ANTIGENICITY OF BRUCELLA CHIMERIC RECOMBINANT PROTEIN BY ELISA
Keywords:
brucellosis, Brucella abortus, Brucella melitensis, chimeric protein, recombinant proteins, antigenicity, indirect ELISA, antibodies, diagnostics.Abstract
The study of the antigenicity of proteins showed that chimeric rOmpBm-Ba and natural proteins of the outer membrane of Brucella have the greatest activity in indirect ELISA. It should be noted that rOmp25 and rOmp31 were not always simultaneously bound to antibodies of the same seropositive animal. In indirect ELISA, chimeric rOmpBm-Ba, compared with rOmp25 and rOmp31, detected the presence of anti-Brucella antibodies by 7.3% and 31.2% more. A comparative study of the diagnostic value of indirect ELISA/rOmpBm-Ba and traditional tests on samples of blood sera of cows subjected to post-slaughter bacteriological research showed a higher reliability of enzyme immunoassay using chimeric recombinant Brucella protein. For example, among animals seropositive for traditional tests, only 62.5% (15/24) were positive in indirect ELISA/OmpBm-Ba, and its results were confirmed in 6 (40%) cases out of 15. The results obtained indicate the advantage of using the chimeric protein rOmpBm-Ba in the serological diagnosis of animal brucellosis compared to recombinant monoproteins.
