OBTAINING OF THE STRAIN PRODUCING RECOMBINANT OMP19 BRUCELLA ABORTUS AND STUDYING OF ITS ANTIGENICITY
Keywords:
brucellosis, outer membrane proteins, recombinant proteins, antigenicity, i-ELISA, diagnostics.Abstract
A strain-producer of Brucella abortus recombinant outer membrane protein (OMP) with a molecular weight of 19 kD (rOMP19) was created and antigenicity of the target product was studied. The recombinant protein was in the cytoplasmic inclusion bodies of the strain-producer E. coli BL21 (DE3), which gave the maximum amount of the desired product by culturing for a day. Comparative
studies of cattle blood sera, positively reacting to brucellosis according to classical serological tests, by indirect enzyme-linked immunosorbent assay (i-ELISA) on the basis of rOMP19, rOMP25 and/or rOMP31, as well as Brucella-derived OMP were carried out. It is concluded that the use of a single recombinant protein in iELISA significantly reduces its sensitivity, therefore effective diagnostic kits may be developed on the basis of combined recombinant proteins
