DETERMINATION OF OPTIMAL CONCENTRATION DIMERETHYLENIMINE FOR INACTIVATION OF STAMM OF "VRC-Z2" OF RABIES VIRUS

Abstract

The results of studying of dimerethyleneimine at final concentrations of 0.1, 0.2 and 0.3 % as for rabies virus of the strain "VRC-RZ2" are given in this paper. As a result of the studies carried out, dimerethylenimine is found to be an effective inactivant when inactivating rabies virus strain "VRC-RZ2." Therefore, for widespread use in the production of inactivated rabies vaccines, we have selected and recommended the inactivantdimerethylenimine at a final concentration of 0.2% at a temperature of (37 ± 0.5) ° C, which for 24 hours completely destroys the infectiousproperties of rabies virus while maintaining high antigenic activity. The developed
inactivation regime in the production of industrial series of rabies vaccines, provides complete inactivation of rabies virus, and we get a safe drug. Determination of residual virulence was carried out on a cell culture of BHK-21/13 for 3 consecutive passages. The results of the studies showed that when the VNK-21/13 cell culture was infected with an inactivated virus suspension within 10 days of observation, there was no manifestation of destructive changes in the monolayer with three consecutivepassaging. When studying the avirulence of inactivated materials on 3-5 day old sucker mice, they remained alive, without showing any clinical signs of the disease throughout the observation period (10 days). The results obtained indicated that the studied samples were avirulent.
Based on the above, it follows that DEI is effective in the inactivation of rabies virus and is recommended for widespread use in the production of inactivated rabies vaccines