BIOLOGICAL PROPERTIES AND MOLECULAR GENETIC CHARACTERISTIC OF THE ZOOPHILIC STRAIN TRICHOPHYTON VERRUCOSUM, ISOLATED FROM HUMAN
Keywords:
dermatomycoses pathogens, dermatomycetes, zoonotic diseases, nutrient media, trichophytosis, arthrospores, mycelium, microconidia, macroconidiaAbstract
The article presents the results of researchers connected with isolation and characterization of the zoophilic strain Trichophyton verrucosum, which caused the infection of the person. As a result of the research, a strain of the fungus, initially identified as Trichophyton verrucosum was obtained, its cultural-morphological, biochemical properties and molecular genetic characteristics were studied. The culture of dermatomycete isolated by us differed by typical cultural properties, had characteristic morphological structures: colorless mycelium, micro- and macroconidia, arthrospores and chlamydospores. At the same time, during the formation of the colony, an unusual polymorphism was noted, usually unusual for T. verrucosum cultures during the initial isolation. Sequencing typing on Sanger allowed to confirm results of laboratory researches. Identification of the fungus by the method of analysis of the ITS region in BLAST obtained 100% confirmation that the culture of the fungus we isolated belongs to the genus Trichophyton, verrucosum species.