SENSITIVITY OF A CLONIC CELL CULTURE POPULATION TESTICULES LAMBS TO LUMPY SKIN DISEASE VIRUS

Abstract

The article presents the results of studies on the sensitivity of various cell cultures to the lumpy skin disease virus. As a biological model, we used primary cultures of kidney cells and testicles of lambs, transplanted passive subpopulations of these cell cultures and transplanted cell lines TT, MDBK, BHK-21, PKEV and Vero. Of the tested cell cultures, the most sensitive were the cell cultures prepared from the testicular tissue of the lambs in the form of primary, passive subculture and clone variants to the lumpy skin disease virus. According to the results, the sensitivity level of the testicular cell culture is close to the indicator activity of the
molecular genetic testing method, in the case of a diagnostic study with a reproductive virus. The cell culture from the testicular tissue of lambs also has high productivity with respect to viral particles of the pathogen of lumpy skin disease. The sensitivity of the TT cell line nodular dermatitis virus in numerical terms was 76.5% and the culture of MDBK cells originating from cattle kidney tissue was 35.3%. Transplanted cell cultures such as BHK-21, PKEV, Vero did not show sensitivity to the lumpy skin disease virus