MOLECULAR MARKERS IN THE DIAGNOSTICS OF OPISTORCHOSIS

Authors

  • A.M.Smagulova, Master of Engineering, Senior Researcher A.B. Bekenova. doctoral student A.V. Katokhin,candidate of biological sciences V.S. Kiyan, PhD,associate professor

DOI:

https://doi.org/10.47100/herald.v1i2.55

Keywords:

opisthorchiasis, metrorchosis, diagnostics, metatserkaria, marita, marker gene, sequencing, phylogenetic tree, primer

Abstract

The authors of this article present the results of a study on the main three marker genes Internal transcribed spacer 1 (ITS1), Internal transcribed spacer 2 (ITS2) и Cytochrome oxidase (СОХ1) used in molecular genetic studies and the creation of diagnostic PCR tests. The peculiarity of the method of DNA extraction from metacercariae and sexually mature forms of maritas is shown. A careful selection of primers was carried out and the main parameters of PCR formulation were worked out. The nucleotide sequences of the O. felineus and M. bilіs
pathogens obtained by sequencing were deposited in the international GenBank database. Based on the obtained nucleotide sequences, phylogenetic trees of closely related organisms of the Opisthorchiidae family were constructed and genetic characteristics of the studied species isolated on the territory of the Republic of Kazakhstan were shown.

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Published

2021-05-20

Issue

No. 2 (105) (2020): Herald of Science of S. Seifullin KAZAKH AGRO TECHNICAL UNIVERSITY

Section

Veterinary sciences Part 2