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Abstract

In this paper, the antigenicity and immunogenicity of natural proteins of the outer membrane of Brucella spp. and its three recombinant proteins have been studied. Comparative studies of blood serum of cattle in the Agglutination Test, Complement Fixation Test, as well as in the Enzyme-linked immunosorbent assay based on the Brucella natural and recombinant proteins were carried out. The results of the studies showed that among heifers seropositive according to the results of classical serological tests specific antibodies to Brucella outer membrane proteins were detected in sera of 90.8% animals by the Enzyme-linked immunosorbent assay. Antibodies to recombinant proteins were found in serum samples of heifers in the range of 21,5% up to 64,6%