mRNA expression and correlation relationships of polymorphic genes identified in different tissues of kalmyk bullocks

Abstract

Background and Aim. The research presented in this article is aimed at investigating the mRNA expression patterns and correlations of polymorphic genes identified in various tissues of Kalmyk bullocks raised at Agrofirma Turk LLP in the Zhambyl region. The aim of the study was to analyze the expression patterns of the ELOVL6 and CRTC2 genes in various tissues of Kalmyk bull calves, identify single nucleotide polymorphisms and assess their correlation with body size traits. The obtained results may serve as a scientific basis for the application of molecular genetic approaches in the breeding of beef cattle.
Materials and Мethods. The study was conducted in 2024 2025. Kalmyk bullocks aged 12-18 months were selected as the object of the study and 13 different tissue samples were collected from three animals of clinically healthy 18-month-old animals. Total RNA was isolated from the tissues and cDNA synthesis was performed. The expression levels of the CRTC2 and ELOVL6 genes were determined using real-time qPCR.
Results. The results showed that the mRNA expression of the CRTC2 and ELOVL6 genes in various tissues of Kalmyk cattle exhibited pronounced tissue-specific patterns. The CRTC2 gene was highly expressed in the heart and liver which are actively involved in energy metabolism, the ELOVL6 gene demonstrated high transcriptional activity in the spleen and intestinal tissues associated with lipid metabolism.
Conclusion. The data obtained suggest that these genes may be considered promising candidate genes associated with meat productivity, helping to clarify their molecular role in the regulation of fat and energy metabolism. The results of the study can serve as a scientific basis for the application of molecular genetic approaches in cattle breeding.