DEVELOPMENT OF ENZYME IMMUNOASSAY FOR SEROLOGICAL DIAGNOSIS OF EQUINE SALMONELLA ABORTION

Abstract

The article describes the results of studies on the development of protocols for the production of enzyme linked immunosorbent assay (ELISA) for the serological diagnosis of Salmonella abortion in mares. The carrier was sensitized using antigens obtained by ultrasonic disintegration and outer membrane proteins (OMPs) of Salmonella enterica abortus equi. Based on the use of the obtained antigens, protocols for setting up an indirect ELISA variant on a 96-well plate were developed. As a result, the optimal parameters for carrying out the reaction were determined and encouraging results were obtained. To determine the effectiveness of the proposed options for setting the ELISA, comparative studies were carried out using a commercial antigen. According to the results of the experiment, a correlation was noted between the data obtained using the compared tests. The developed version of the enzyme immunoassay based on the use of the OMB antigen can be proposed for implementation in veterinary practice, since today there is no similar domestic test.