THE USE OF RECOMBINANT CAMPYLOBACTER JEJUNI ANTIGENS FOR THE PRODUCTION OF SPECIFIC POLYCLONAL ANTIBODIES
DOI:
https://doi.org/10.51452/kazatu.2022.2(113).1064Keywords:
Campylobacter jejuni; campylobacteriosis; recombinant antigen; specific antibodies; antibody titer; diagnostics; immunochromatographic test.Abstract
The article presents data on the production of specific polyclonal antibodies to recombinant antigens of Campylobacter jejuni, which were previously synthesized proteins of the outer membrane Omp18 and MOMP of Campylobacter. The optimal scheme for immunization of experimental animals was determined, which made it possible to stimulate the body to produce specific antibodies against the original antigen in high titers. ELISA testing made it possible to establish the maximum titers, to the Omp18 protein - 1:102400, and to MOMP - 1:204800. The resulting antibodies were isolated from serum and purified by chromatography. Their main properties were studied, which showed that these immunoglobulins can be used in the design of an immunochromatographic test for the rapid detection of campylobacteriosis pathogens in food products of animal origin and other biological objects.
