THE STUDYING OF THE GALLERIA MELLONELLA LARVAE GUT MICROBIOME AND DETERMINING ITS SPECIES COMPOSITION
Keywords:Galleria mellonella, pest, larvae, gut microbiome, molecular genetic characteristics, cultivation, 16S rRNA sequencing.
Galleria mellonella is common pest of honeybee hives and can be found everywhere where beekeeping is practicing with the exception of regions with a harsh climate and high attitude. Galleria mellonella is one of the common testing organism in
investigation of several pesticides, insect pathogens and biological active substances. However, most properties of this organism remain unstudied. Immunity and high surviving abilities of organisms closely related to their microbiome and symbiosis.
Digestive tract (gut) of Galleria mellonella had been studied to obtain knowledge about its microbiological composition via microbiology and molecular genetic analysis techniques. Gut microbiome was habitat to various bacterial species, mostly
to Bacillus genus. Intestine microbiome of larvae populated with enormous amount and types of bacteria gives its host several abilities to digest range of different material and playing defensive role from foreign gut intruders by secreting enzymes
and antibacterial substances. Investigation of mentioned microorganisms from gut were studied and characterized.