OBTAINING EXCRETORY-SECRETARY AND SOMATIC ANTIGENS OF TRICHINELLA SPIRALIS AND DETERMINATION OF ANTIGENIC PROPERTIES

Abstract

Trichinellosis still poses a threat to the life and health of animals and people. Despite all its study, early diagnosis when parasite larvae enter the body does not show signs of invasion into the intestinal stage. Enzyme-linked immunosorbent assay reveals the presence of the parasite at 2-4 weeks after invasion, when in adults the larvae are already encapsulated in the muscles. To date, the most accurate method is ELISA using excretory-secretory and somatic antigens. The detection revealed excretory-secretory and somatic antigens to the most common detection of Trichinella spiralis isolated from carcasses of wild animals. As a result of electrophoresis, a certain composition of ES-Ag was revealed, the molecular weights of which vary from 45 to 100 kDa, and C-Ag with molecular weights from 25 to 300 kDa. Immunoblotting revealed valuable diagnostic isolations of the 15 kDa excretory-secretory protein, the 300 kDa somatic antigen, which are tested with the blood serum immunoglobulins of experimentally infected animals. According to the results of ELISA, the presence of specific antibodies to the obtained antigens was revealed in the group of rabbits infected with larvae of capsular T. spiralis, detected from 14 days after the detection of a pronounced growth of specific antibodies, however, on day 70, the antibody titer was high 1:800 to 1:12800. Thus, the use of excretory-secretory antigen makes it possible to determine the presence of invasion from the 14th day after infection.